Journal: Experimental and Therapeutic Medicine

Article Title: Long non-coding RNA LUCAT1 promotes the progression of clear cell renal cell carcinoma via the microRNA-375/YAP1 axis

doi: 10.3892/etm.2021.10186

Figure Lengend Snippet: Expression of LUCAT1 and YAP1 in ccRCC tissues and cells. (A) The expression of LUCAT1 in 62 pairs of ccRCC tissues and adjacent healthy tissues was detected by RT-qPCR. (B) The association between the expression level of LUCAT1 and the overall survival in patients with ccRCC was determined by Kaplan-Meier analysis. (C) The expression of YAP1 in 62 pairs of ccRCC tissues and adjacent healthy tissues was detected by RT-qPCR. (D) The correlation between LUCAT1 and YAP1 expression level in ccRCC tissues was assessed using Pearson's correlation analysis. The expression level of (E) LUCAT1 and (F) YAP1 in the normal human proximal tubular epithelial cell line HK-2 and RCC cell lines (786-O, Caki-1, A498, 769-P and ACHN) was determined by RT-qPCR. * P<0.05 and ** P<0.01 vs. healthy tissues or normal cells. RT-qPCR, reverse transcription-quantitative PCR; LUCAT1, lung cancer-associated transcript 1; YAP1, yes-associated protein 1; ccRCC, clear cell renal cell carcinoma.

Article Snippet: A normal human proximal tubular epithelial cell line (HK-2) and RCC cell lines (786-O, Caki-1, A498, 769-P and ACHN) were obtained from American Type Culture Collection.

Techniques: Expressing, Quantitative RT-PCR, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Experimental and Therapeutic Medicine

Article Title: Long non-coding RNA LUCAT1 promotes the progression of clear cell renal cell carcinoma via the microRNA-375/YAP1 axis

doi: 10.3892/etm.2021.10186

Figure Lengend Snippet: LUCAT1 directly binds to miR-375. (A) The binding sites between LUCAT1 and miR-375 were predicted using the lncBase Predicted v.2 software. (B) The effects of miR-375 overexpression on the luciferase activity of LUCAT1-WT or LUCAT1-MUT reporter were detected using a dual-luciferase reporter assay. (C) miR-375 expression in 62 pairs of ccRCC tissues and adjacent healthy tissues was measured by RT-qPCR. (D) The correlation between LUCAT1 and miR-375 expression levels in ccRCC tissues was analyzed by Pearson's correlation analysis. (E) miR-375 expression in the normal human proximal tubular epithelial cell line HK-2 and RCC cell lines (786-O, Caki-1, A498, 769-P and ACHN) was measured by RT-qPCR. (F) Overexpression or (G) knockdown efficiency of LUCAT1 was determined by RT-qPCR in ACHN and 769-P cells or Caki-1 and A498 cells, respectively. The effects of LUCAT1 (H) overexpression or (I) knockdown on miR-375 level in ACHN and 769-P cells or Caki-1 and A498 cells, respectively, were measured by RT-qPCR. ** P<0.01 vs. the indicated groups or HK-2 cells. RT-qPCR, reverse transcription-quantitative PCR; LUCAT1, lung cancer-associated transcript 1; RCC, renal cell carcinoma; ccRCC, clear cell RCC; miR, microRNA; WT, wild-type; MUT, mutant; si, small interfering; NC, negative control.

Article Snippet: A normal human proximal tubular epithelial cell line (HK-2) and RCC cell lines (786-O, Caki-1, A498, 769-P and ACHN) were obtained from American Type Culture Collection.

Techniques: Binding Assay, Software, Over Expression, Luciferase, Activity Assay, Reporter Assay, Expressing, Quantitative RT-PCR, Knockdown, Reverse Transcription, Real-time Polymerase Chain Reaction, Mutagenesis, Negative Control

Journal: Experimental and Therapeutic Medicine

Article Title: Long non-coding RNA LUCAT1 promotes the progression of clear cell renal cell carcinoma via the microRNA-375/YAP1 axis

doi: 10.3892/etm.2021.10186

Figure Lengend Snippet: YAP1 is a direct target of miR-375 in RCC cells. (A) Predicted binding sites between miR-375 and YAP1-WT or MUT sites in YAP1-MUT reporter. (B) The effects of miR-375 overexpression on the luciferase activity of YAP1-WT or YAP1-MUT reporter were determined using a dual-luciferase reporter assay. (C) Pearson's correlation analysis was performed to assess the correlation between miR-375 and YAP1 expression levels in clear cell RCC tissues. The effects of miR-375 (D) overexpression or (E) knockdown on YAP1 protein expression were examined using western blot assay in Caki-1 and A498 cells. ** P<0.01. YAP1, yes-associated protein 1; RCC, renal cell carcinoma; miR, microRNA; WT, wild-type; MUT, mutant; NC, negative control.

Article Snippet: A normal human proximal tubular epithelial cell line (HK-2) and RCC cell lines (786-O, Caki-1, A498, 769-P and ACHN) were obtained from American Type Culture Collection.

Techniques: Binding Assay, Over Expression, Luciferase, Activity Assay, Reporter Assay, Expressing, Knockdown, Western Blot, Mutagenesis, Negative Control